Gel Electrophoresis and Transilluminator

User manual chapter for the Bento Lab gel electrophoresis and transilluminator module. Includes a physical specification, information on casting gels and connecting the gel box, and operation guide for the interface.

Specifications

Voltage 50V – 120V
Box Dimensions91mm x 78mm
Visualisation470nm blue light and diffuser
Viewing WindowAs box dimensions
FilterAmber acrylic

Before Use: Preparing the gel and samples

Use the plastic shutters to create buffer zones before you pour the gel.

Prepare the gel in a beaker. Once it is not too hot to hold for 30 seconds, add the safe stain and pour into the gel box. Add the comb. Allow the gel to cool. In the meantime, prepare your samples. Once the gel has solidified, remove the comb. Remove the shutters. Pipette the samples into the well and add the buffer.

Gel box with comb and shutters for buffer zones

Running Gel Electrophoresis

Slide the gel lid onto the gel box carefully, then connect the leads of the gel box lid into the Bento Lab Gel Electrophoresis power supply.

The gel box slides sideways.
Red and black leads plug into Bento Lab

 On the interface, select the Gel Electrophoresis module from the status screen. On this screen the voltage can be set and a timer for the Gel Electrophoresis power supply can be set. 

 The blue light transilluminator can also be turned un with the light bulb button.

Main Gel Electrophoresis screen

 To start Gel Electrophoresis, set the voltage and click on the timer button. Note that since Bento Lab’s gel box is a mini-gel, 50V will be sufficient for most applications.

 The power supply will start, once the timer has been confirmed.

Setting a timer for Gel Electrophoresis