TBE Electrophoresis Buffer

Name: TBE Electrophoresis Buffer
SKU: 4295
Availability: InStock

Buffer for gel electrophoresis (Tris-borate-EDTA)

Price range: £5.45 through £39.50 ex VAT

TBE Electrophoresis Buffer

Buffer for gel electrophoresis (Tris-borate-EDTA)

Price range: £5.45 through £39.50 ex VAT

Prepare solutions for gel electrophoresis quickly and accurately with this concentrated TBE buffer. Once diluted, the 1L concentrated stock solution provides enough buffer to cast and run 400 gels with Bento Lab.

Safety Data Sheet

Description

Prepare solutions for gel electrophoresis quickly and accurately with this concentrated TBE buffer. TBE Buffer (Tris-borate-EDTA) is one of the most commonly used buffers for DNA and RNA gel electrophoresis. This buffer is perfect for use with Bento Lab. The buffer can be used to cast gels, and as running buffer for gel electrophoresis.

Once diluted, the 1L concentrated stock solution provides enough buffer to cast and run 400 gels with Bento Lab. Dilute to a 0.5X solution by diluting 20x in water.

Details

Easy to use. No weighing messy powder, dilute and go.
Saves time. Avoid waiting for powder to dissolve with a ready-made 10X concentration solution.
Saves space. Concentrated stock minimises the space needed for storage.

Applications

PCR product analysis
Gel extraction and DNA recovery
Plasmid characterisation
Southern/Northern blotting
DNA Fingerprinting
RFLP analysis
Detection of DNA polymorphisms
Restriction enzyme mapping
Glycoprotein Electrophoresis

Specifications

Recommended usage

Make a working concentration buffer of 0.5x TBE for agarose gel electrophoresis by adding 50 mL of 10x TBE to 950 mL of distilled or deionised water.

Use 0.5x TBE buffer for casting and running agarose gels with Bento Lab or other small minigel electrophoresis systems.

Components

1x solution contains 89 mM Tris, 89 mM borate, 2 mM EDTA, pH 8.2-8.4

Storage & Stability

Store at room temperature.

Some crystallisation may occur after long storage or cold storage. If heavy crystallisation occurs, transfer the buffer to a beaker and heat until crystals redissolve.

Shipping conditions

Shipped at room temperature.

Resources

Protocol: Casting a gel

FAQ

Crystals have formed in my 10x TBE buffer – what should I do?

Concentrated stock solutions like 10x TBE buffer can form a crystalline precipitate over time or in colder conditions. This is entirely normal and does not affect the composition of the buffer chemicals. Cloudiness or a few small crystals will disappear when diluted to 0.5x or 1x working concentrations.

To remove heavy crystalisation, heat the 10x TBE buffer at 40°C–60°C until the crystals redissolve. Very heavy crystallisation may require increased temperatures or increased time to redissolve.

If your 10x TBE buffer is in a 50 mL tube, add hot water to a beaker, and place your 50 mL tube in the beaker until the crystals dissolve. Occasional shaking of the tube can speed up crystal dissolution. Alternatively, pour out the 10x TBE buffer and crystals into an empty beaker, microwave it in 30 s blasts until the crystals dissolve, and pour it back in after cooling.

If you have a 1 L bottle of 10x TBE, transfer all liquids and crystals into beakers and carefully microwave in short (e.g. 30 s) bursts until the buffer is hot. Gently swirl until the crystals dissolve (this may take some time for heavy crystallisation).