100 bp DNA Ladder

Ready-to-use molecular weight markers for gel electrophoresis

£18.00£35.00 ex VAT

100 bp DNA Ladder

Ready-to-use molecular weight markers for gel electrophoresis

£18.00£35.00 ex VAT

Estimate the size of DNA fragments of sizes 100 – 3,000 bp during gel electrophoresis using a 100 bp DNA Ladder. Provided as a mix of DNA fragments at known sizes and concentrations, a 500 µL tube provides enough ladder to run 100 lanes.

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Description

DNA Ladders, also known molecular-weight size markers, contain a set of predetermined DNA fragment sizes. These markers are a set of standards used to identify the approximate size and concentration of a DNA fragment run on gel electrophoresis.

This ready to load 100 bp ladder is ideal for standard use during the electrophoresis of PCR-amplified DNA.

Details

  • Contains known sized DNA fragments to allow comparison of the size and concentration of sample DNA fragments.
  • Contains loading dye for easy pipetting into electrophoresis gel wells, and as a visual aid to track the extent of DNA migration during gel electrophoresis.
  • Stable at room temperature, and can be stored up to nine months at room temperature without significant degradation.
  • Safe to use, containing no substances that are considered hazardous to health in their given concentrations

Applications

Gel Electrophoresis

Specifications

Recommended Usage

Recommended for routine use during gel electrophoresis of DNA samples containing fragments of 100 – 3,000 bp in size.

For best resolution and accuracy use 5 µL of DNA ladder in the electrophoresis gel well to the left of the first sample, and the well to the right of the last sample. For example, the first and last wells if all wells are being used. No loading dye is needed because it is already included in the DNA ladder solution.

Components

100 bp Ladder (13 DNA fragments at 0.1 µg/µL (size range: 100 – 3000 bp), 10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, 0.17% SDS). 

Storage & Stability

Store at room temperature for up to nine months, or at –20 °C for longer term storage.

Shipping conditions

Shipped at room temperature.

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